Thymosin alpha-1 (a1) ELISA Assay Kit

$1,500.00

The Thymosin alpha-1 (a1) ELISA Assay Kit is an enzyme immunoassay intended for the quantitative determination of thymosin α1 in serum and thymus extract. For research use only. Not for use in diagnostic procedures.

SKU: KR9510 Categories: , ,

Thymosin alpha-1 (a1) ELISA Assay Kit

Thymosin alpha-1 ELISA Assay Kit manufactured in Germany by Immundiagnostik

Size: 1×96 wells
Standard Range: 4-1000 ng/ml
Incubation Time: 18h; 1.5h; 1h; 10-20min
Sample Type: Serum, Thymus extract
Sample Size: 400 µl
Alternative Names: Human Thymosin alpha-1 ELISA, Human Thymosin a1 ELISA, Thymosin α1 ELISA, TMS alpha 1 ELISA Assay Kit
For Research Use Only
Controls Included


Assay Principle

This Thymosin alpha-1 (a1) ELISA is designed for the quantitative determination of thymosin a1 (Tα1). In this test, polyclonal rabbit antibodies directed against synthetic Tα1 are used. The test principle is based on a competition between antigen in the sample or standards and the antigen coated on the wells of microplate. A peroxidase-conjugated anti-body is used for detection and quantification, and tetramethylbenzidine (TMB) as a peroxidase substrate. The enzymatic reaction is terminated by an acidic stop solution. A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from standard. Tα1, present in the samples, is determined directly from this curve.


Quality Control
We recommend the use of external controls for internal quality control, if possible. Control samples should be analysed with each run. Results, generated from the analysis of control samples, should be evaluated for acceptability using appropriate statistical methods. The results for the samples may not be valid if within the same assay one or more values of the quality control sample are outside the acceptable limits.
Reference range
The values in healthy people are strongly age-dependent. Newborns show the highest values, and from the age of 20 onwards the serum values are falling. We recommend each laboratory to establish its own reference range for different age ranges.


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Additional Information

Assay Background


Thymosin α1 was the first single peptide isolated from thymus fraction 5. It acts on T-helper and NK-cells. Thymosin α1 has been reported for exert effects on hormon regulating the hypothalamus (1). Thymosin α1 has been demonstrated to have beneficial effects in animal models of liver and colon carcinoma (2) or leukaemia (3). Its use as a prognostic factor in human studies, e.g. colon carcinoma (4) has been discussed. Thymosin α1 has been successfully used as component combined chemo-therapy in bronchial carcinoma (5).

Manual

Product Manual