Soluble Mannose Receptor ELISA


The Human Soluble Mannose receptor (sMR) ELISA Kit is to be used for the quantitative determination of sMR in plasma samples. The Eagle Biosciences Human Soluble Mannose receptor (sMR) ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

Soluble Mannose Receptor ELISA

The Soluble Mannose Receptor ELISA For Research Use Only

Sizes: 1×96 wells and 2×96 wells
Sensitivity: 1 ng/ml
Standard Range: 1 to 200 ng/ml
Incubation Time: 3.5 hours
Sample Type: Plasma
Sample Size: 100 µl

Assay Background

Recent studies have indicated increased concentrations of the soluble form of Mannose Receptor (MR) in plasma of patients with infection diseases such as sepsis, and acute hepatitis. MR’s are especially expressed on cell membranes of macrophages and function as Pattern Recognition Receptors (PRR). Exact function and (in-vivo) shedding-mechanism of the soluble form, soluble Mannose Receptor (sMR) is still unknown. Increased sMR’s in plasma can be due to some pathogens in the body which stimulate the (in-vivo) shedding mechanism of the MR’s. sMR’s which are bound to the mannose residues of these pathogens, prevent it from being recognized and phagocyted by MR’s. As a result, the innate immunity is slowly suppressed and a vicious circle (which increases the sMR’s in plasma) is created. The body does this probably to partly slow the innate immunity to prevent going out of balance. When the disease being treated, the pathogens which are present in the body of the patient, will be less and less . In the body becoming less cleaved mannose receptors and the sMR in plasma will gradually decrease. The measurement of sMR’s in plasma may be important for the diagnoses/monitoring of diseases such as sepsis, pneumonia and acute hepatitis.

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Additional Information

Assay Principle

The sMR ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing sMR. Biotinylated tracer antibody will bind to the captured sMR. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the sMR standards (log). The sMR concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.


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