SeroCP Semi-quantitative IgG ELISA Assay
The SeroCP Semi-quantitative IgG ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: Cut-Off Control
Dynamic Range: 0-180 pmol/l
Incubation Time: 2.25 hours
Sample Type: Serum
Sample Size: 10 µl
Sensitivity and Specificity
The Eagle Biosciences SeroCP™ Quant IgG was evaluated against the SeroCP IgG (Catalog No. 191-01) The study used 197 sera samples from symptomatic individuals and 34 sera samples from healthy individuals.
Sensitivity and specificity were calculated:
Sensitivity: 190/197 = 96.4%
Specificity: 33/34 = 97%
The Eagle Biosciences SeroCP™ Quant Plates are supplied coated with purified elementary bodies of C.pneumoniae (TWAR – 183) antigens. The serum to be tested is diluted and incubated in the SeroCP™ Quant plate for 1h at 37°C. In this step C.pneumoniae antibodies are bound to the immobilized antigens. Non-specific antibodies are removed by washing. Anti-human IgG conjugated to Horseradish Peroxidase (HRP) is added and incubated 1h at 37°C. In this step the HRP-Conjugate is bound to the prebound antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB-Substrate, the Substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced Substrate. Upon the addition of the Stop Solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620nm. The absorbance is proportional to the amount of the specific antibodies that are bound to the coated antigens.