SeroCP Semi-quantitative IgA ELISA Assay
The SeroCP Semi-quantitative IgA ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: Cut-Off Control
Incubation Time: 2.25 hours
Sample Type: Serum
Sample Size: 10 µl
Alternative Name: Chlamydia pneumoniae, quant c. pneumoniae
Sensitivity and Specificity
The Eagle Biosciences SeroCP Quant IgA was evaluated against the SeroCP IgA (Catalog No. 193-01). The study used 230 sera samples from symptomatic individuals and 58 sera samples from healthy individuals.
Sensitivity and specificity were calculated:
Sensitivity: 218/230 = 94.8%
Specificity: 54/58 = 93.1%
The Eagle Biosciences SeroCP Quant Plates are supplied coated with purified elementary bodies of C.pneumoniae (TWAR 183) as antigens. The serum to be tested is diluted and incubated in the SeroCP Quant plate for 1h at 37°C. In this step C.pneumoniae antibodies are bound to the immobilized antigens. Non-specific antibodies are removed by washing. Anti-human IgA conjugated to Horseradish Peroxidase (HRP) is added and incubated 1h at 37°C. In this step the HRP-Conjugate is bound to the prebound antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB-Substrate, the Substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced chromogen. Upon the addition of the Stop Solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620nm. The absorbance is proportional to the amount of the specific antibodies that are bound to the coated antigens.