SeroCP RT C. pneumoniae IgA ELISA Assay
The SeroCP RT C. pneumoniae IgA ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: Cut-Off Control
Incubation Time: 1.5 hours
Sample Type: Serum
Sample Size: 10 µl
The Eagle Biosciences SeroCP RT Plates are coated with C. pneumoniae specific antigens. Serum to be tested is diluted and incubated with the pre-coated SeroCPTM RT plate 30 minutes at Room Temperature (RT). In this step, C. pneumoniae specific antibodies are bound to the immobilized C. pneumoniae specific antigens. Non-specific antibodies are removed by washing. Anti-human IgA conjugated to horseradish peroxidase (HRP) is added and incubated 30 minutes at Room Temperature. In this step, the HRP-conjugate is bound to the pre-formed antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620 nm. The absorbance is proportional to the amount of the specific antibodies which are bound to the immobilized C. pneumoniae specific antigens.