SeroCP RT C. pneumoniae IgM ELISA Assay


The SeroCP IgM (RT) ELISA Assay Kit is an Enzyme Linked Immunosorbent Assay (ELISA) test for the determination of IgM antibodies specific to Chlamydia pneumoniae in human serum. The test enables early diagnosis of current infection in a single serum specimen by determination of IgM antibodies. This SeroCP IgM (RT) ELISA Assay Kit is a new configuration of ELISA test which presents advantageous features: incubations at ambient temperature; short test duration; and utilizing ready-to-use conjugate. The Eagle Biosciences SeroCP IgM (RT) ELISA Assay Kit is for Research Use Only and is not intended for diagnostic or therapeutic purposes.

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SeroCP RT C. pneumoniae IgM ELISA Assay

The SeroCP RT C. pneumoniae IgM ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: Cut-Off Control
Incubation Time: 1.5 hours
Sample Type: Serum
Sample Size: 10 µl

Assay Principle

The Eagle Biosciences SeroCP RT Plates are coated with C. pneumoniae specific antigens. Serum to be tested is diluted and incubated with the pre-coated SeroCPTM RT plate 30 minutes at Room Temperature (RT). In this step C. pneumoniae specific antibodies are bound to the immobilized C. pneumoniae specific antigens. Non-specific antibodies are removed by washing. Anti-human IgM conjugated to horseradish peroxidase (HRP) is added and incubated 30 minutes at Room Temperature. In this step the HRP-conjugate is bound to the pre-formed antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620 nm. The absorbance is proportional to theamount of the specific antibodies which are bound to the immobilized C. pneumoniae specific antigens.

Related Products

SeroCP C. pneumoniae IgM ELISA Assay Kit
SeroELISA Chlamydia True IgM Assay
SeroMP Recombinant Mycoplasma pneumoniae IgM ELISA Assay Kit

Additional Information

Assay Background

Chlamydia pneumoniae (TWAR183) is an emerging infectious agent with a spectrum of clinical manifestations, including upper and lower respiratory tract infections. The majority of C. pneumoniae infections are mild and asymptomatic yet, may cause serious diseases, such as pharyngtitis, sinusitis, acute bronchitis and community acquired pneumonia. Undetected and untreated infection may lead to prolonged and persistent disease. Recent data indicates a possible association between C. pneumoniae infection and chronic diseases.

Seroprevalence of C. pneumoniae among children is low but increases sharply until middle age, where after it remains high. Difficulties in sample collection and inaccessibility of the infected site seriously affect the usefulness of direct detection methods. Therefore, serological testing is routinely used and serves as a non-invasive tool in identification of both distal and chronic chlamydial infections, where direct detection methods are rarely efficient. In addition, the presence of certain antibody types may also indicate the state of the disease.

Primary chlamydial infection is characterized by a predominant IgM response within 2 to 4 weeks and a delayed IgG and IgA response within 6 to 8 weeks. After acute C. pneumoniae infection, IgM antibodies are usually lost within 2 to 6 months, IgG antibody titers usually decrease slowly; whereas IgA antibodies tend to disappear rapidly. When primary chlamydial infection is suspected, the detection of IgM is highly diagnostic. However, in recurrent or chronic infections the prevalence of IgM is low and therefore absence of IgM does not necessarily exclude on-going infection. In reinfection, IgG and IgA levels rise quickly, often in one to two weeks.

IgA antibodies have shown to be a reliable immunological marker of primary, chronic and recurrent infections. These antibodies usually decline rapidly to baseline levels following treatment and eradication of the chlamydia infections. The persistence of elevated IgA antibody titers is generally considered as a sign of chronic infection.

IgG antibodies persist for long periods and decline very slowly. Therefore, the presence of IgG antibodies is mainly indicative of a chlamydia infection at an undetermined time. However, a four-fold rise in IgG or high levels of IgG antibodies may indicate an on-going chronic infection. SeroCP RT is an ELISA based assay in which purified elementary bodies of C. pneumonaie (TWAR-183) are used as antigens to detect the antibody response in humans. For complete diagnosis of current, chronic or past infections, it is recommended to determine IgG, IgM and IgA antibodies to C. pneumoniae.


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