SARS-CoV-2 Nucleoprotein Qualitative ELISA Assay Kit


The SARS-CoV-2 Nucleoprotein Antigen ELISA Assay Kit is used as an analytical tool for qualitative antigen determination of Human SARS-CoV-2 (Covid-19) in respiratory specimens and human serum. The Eagle Biosciences SARS-CoV-2 Nucleoprotein Antigen ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

SARS-CoV-2 Nucleoprotein Qualitative ELISA Assay Kit

The SARS-CoV-2 Nucleoprotein Qualitative ELISA Assay Kit is For Research Use Only

Size: 12×8 wells
Sensitivity: 42.5 pg/mL
Incubation Time: 3 hrs 20 mins
Sample Type: Respiratory specimens, and serum
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19

Assay Principle

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human SARS-CoV-2 (Covid-19) present in the sample are bound by the capture antibodies. After incubation the wells are washed and followed by HRP- conjugated Detection Antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human SARS-CoV-2 (Covid-19) in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Sample Preparation and Storage
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.
1. Extract as soon as possible after specimen collection as per relevant procedure. The samples should be tested as soon as possible after the extraction. Alternately the extracted samples can be kept in -20°C. Avoid repeated freeze-thaw cycles.
2. Serum- Coagulate at room temperature for 10-20 minutes; centrifuge for 20-min at 2000-3000 rpm. Remove the supernatant. If precipitation appears, recentrifuge.
3. Respiratory Specimens- Collect sample in a sterile container. Centrifuge for 20-mins at 2000-3000 rpm. Remove the supernatant carefully. It is recommended to follow the CDC (Centre for Disease Control), Atlanta, USA guidelines for specimen handling and treatment.

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Additional Information

Assay Background

The SARS-CoV-2 (Covid-19) Nucleoprotein Antigen Qualitative ELISA Assay Kit is used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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