SARS-CoV-2 Spike Protein Antigen Qualitative ELISA Assay Kit


The SARS-­CoV-­2 Spike Protein Antigen ELISA kit is used as an analytical tool for qualitative antigen determination of Human SARS-­CoV-­2 spike proteins in human serum and plasma and cell culture supernatant. The Eagle Biosciences SARS-­CoV-­2 Spike Protein Antigen ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

SARS-CoV-2 Spike Protein Antigen Qualitative ELISA Assay Kit

The SARS-CoV-2 Spike Protein Antigen Qualitative ELISA Assay Kit is For Research Use Only

Size: 12×8 wells
Sensitivity: 6.8 ng/mL
Incubation Time: 3 hrs
Sample Type: Serum, plasma, and cell culture supernatants
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19

Assay Principle

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-­coated onto microwells. Samples and standards are pipetted into microwells and Human SARS-­CoV-­2 (Covid-­19) spike proteins present in the sample are bound by the capture antibodies. After incubation the wells are washed and followed by HRP-­ conjugated Detection Antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-­specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human SARS-­CoV-­2 (Covid-­19) spike proteins in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.”

Sample Preparation and Storage
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.

Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Repeated freezing and thawing should be avoided. If samples are to be used for several assays, initially aliquot samples and keep at -20°C.

Samples should be diluted 1:1000 (v/v) for optimal recovery, (for example 1 ul sample + 999 ul sample diluent) prior to assay. In cases where matrix interferences is under or over observed, the samples may be diluted with Sample Diluent accordingly.

The samples may be kept at 2 – 8°C for up to three days. For long-term storage please store at -20°C.

Note: Grossly hemolyzed samples are not suitable for use in this assay

Cell Culture Supernates -­ Remove particulates by centrifugation and assay immediately or aliquot and store samples at -­20℃ or lower temperature. Avoid repeated freeze-­thaw cycles. If the use of original supernate sample or low dilution (<5 fold) are necessary due to the expected low concentration of antigen supernates need be adjust to neutral pH condition before assay.

Related Products

Mouse SARS-CoV-2 Spike RBD Antibody Quantitative ELISA
SARS-CoV-2 Spike Protein Antigen Quantitative ELISA
SARS-CoV-2 Spike RBD Antigen Quantitative ELISA

Additional Information

Assay Background

The SARS-CoV-2 Spike Protein Antigen Qualitative ELISA Assay Kit is used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations