SARS-CoV-2 Spike Protein S2 Antigen Quantitative ELISA Assay Kit


The SARS-CoV-2 Spike Protein S2 Antigen ELISA kit is used as an analytical tool for quantitative antigen determination of Human SARS-CoV-2 spike protein S2 in human serum and plasma and cell culture supernatant. The Eagle Biosciences SARS-CoV-2 Spike Protein S2 Antigen ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

SARS-CoV-2 Spike Protein S2 Antigen Quantitative ELISA Assay Kit

The SARS-CoV-2 Spike Protein S2 Antigen Quantitative ELISA Assay Kit is For Research Use Only

Size: 12×8 wells
Sensitivity: 6.8 ng/ml
Standard Range: 0-500 ng/ml
Incubation Time: 3 hrs
Sample Type: Serum, plasma, and cell culture supernatants
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19

Assay Principle

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human SARS-CoV-2 (Covid-19) spike protein S2 present in the sample are bound by the capture antibodies. After incubation the wells are washed and followed by HRP- conjugated Detection Antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human SARS-CoV-2 (Covid-19) spike proteins in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Sample Preparation and Storage
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation. Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided. Samples should be diluted 1:5000 (v/v) for optimal recovery, (for example 1 ul sample + 4999 ul sample diluent) prior to assay. In cases where matrix interferences is under or over observed, the samples may be diluted with Sample Diluent accordingly. The samples may be kept at 2 – 8°C for up to three days. For long-term storage please store at -20°C.
Note: Grossly hemolyzed samples are not suitable for use in this assay
Cell Culture Supernates – Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃ or lower temperature. Avoid repeated freeze-thaw cycles. If the use of original supernate sample or low dilution (<5 fold) are necessary due to the expected low concentration of antigen supernates need be adjust to neutral pH condition before assay. Note: The sample should be diluted to within the working range of the assay in 1X Assay Diluent. The exact dilution must be determined based on the concentration of specific target in individual samples.

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Additional Information

Assay Background

The SARS-CoV-2 (Covid-19) Spike Protein S2 Antigen ELISA Assay Kit is used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.
The spike protein (S-protein) of the SARS-CoV-2 virus is the common target for neutralizing antibodies and vaccines. Spike protein contains two subunits, S1 and S2. S1 contains a receptor binding domain (RBD), which is responsible for recognizing and binding with the cell surface receptor. S2 subunit contains other basic elements needed for the membrane fusion. SARS-CoV-2 (2019-nCoV) (ref.) can infect the human respiratory epithelial cells through interaction with human ACE2. Indeed, the recombinant Spike protein can bind with recombinant ACE2 protein.

Typical Standard Curve

Package Inserts

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