Recombinant SARS-CoV-2 Spike Glycoprotein CHO
The Recombinant SARS-CoV-2 Spike Glycoprotein CHO is For Research Use Only
Description: Recombinant Human Coronavirus SARS-CoV-2 Spike Protein S1 subunit, Receptor-Binding
Domain (RBD), NCBI Accession Number: MN908947
Expression System: CHO cells
Tag: HIS Tag C-Terminus
Purification: Affinity chromatography
Extinction Coefficient: 33850 M-1.cm-1 Abs 0.1% (=1 g/l) 1.29 assuming all pairs of Cys residues form cystines
Predicted Molecular Weight: 26.1 kDa
SDS Page: Approx. 30 kDa
Concentration: 1 mg/ml
Purity: >95% by SDS PAGE
Endotoxins: Negative on cell-based assay
Activity: Active form: positive binding with recombinant ACE2 Human. Recognized by CR3022 anti RBD recombinant antibody and Covid-19 positive patients serum samples.
Stability and Storage: Store at -20°C. Avoid repeated freeze-thaw cycles. Stable one week at 2 to 8°C after reconstitution under sterile conditions. Stable 12 months -20°C to -80°C after reconstitution under sterile condition.
Alternative Names: SARS-CoV S, RBD, S protein, Glycoprotein S1
Spike protein (S protein) is one of four structural proteins of Coronavirus (SARS-Cov, SARS-Cov-2, MERS amongst other), S protein plays the most important role in viral attachment, fusion and entry, and it serves as a target for development of antibodies, entry inhibitors and vaccines.
In the S protein, the Receptor Binding Domain (RBD) mediates viral entry of SARS-Cov and SARS-Cov-2 C into host cells by its interaction with the membrane receptor ACE2 (angiotensin-converting enzyme 2).
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