Rat Prealbumin ELISA Assay


The Rat Prealbumin ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Prealbumin in biological samples of rat.

Rat Prealbumin ELISA Assay

The Rat Prealbumin ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 4.508 ng/ml
Dynamic Range: 15.625 ng/ml – 500 ng/ml
Incubation Time: 60 minutes
Sample Type: Plasma, Serum
Sample Size: 100 μL

Assay Principle

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Prealbumin present in samples reacts with the anti-Prealbumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-PRE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound PRE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of PRE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of PRE in the test sample. The quantity of PRE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles.

Related Products

Mouse Prealbumin ELISA Kit
Human Prealbumin ELISA Assay
Rat Albumin Assay Kit

Additional Information

Assay Background

Prealbumin (PRE) is a tetrameric protein consisting of 4 identical polypeptide chains. It migrates more rapidly than serum albumin on electrophoresis. It can be used as a marker the nutritional evaluation of patients with a variety of diseases.

Typical Standard Curve

Rat Prealbumin ELISA Assay


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