Canine C3 ELISA Assay


The Eagle Biosciences Canine (Dog) C3 ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring C3 in biological samples of dogs.

SKU: CC341-K01 Categories: , ,

Canine C3 ELISA Assay

The Canine C3 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.947 ng/ml
Standard Range: 6.25 – 200 ng/ml
Incubation Time: 50 minutes
Sample Type: Serum, Plasma
Sample Size: 100 µl
Alternative Names: Complement 3, Complement C3

Assay Principle

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the C3 present in samples reacts with the anti-C3 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-C3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of C3 in the test sample. The quantity of complement 3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Blood should be collected by venipuncture and the serum separated from the cells, after clot formation, by centrifugation. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Specimens may be shipped at room temperature and then stored refrigerated at 2-8°C if testing is to take place within one week after collection. If testing is to take place later than one week, specimens should be stored at -20°C. Avoid repeated freeze-thaw cycles.

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Additional Information

Assay Background

A number of serum proteins participate in acute inflammatory reactions. These include the complement, coagulation and kinin systems as well as a number of other proteins, known as acute phase proteins, that regulate acute inflammation. The complement system is a complex set of up to 20 serum proteins. The most abundant and pivotal of the complement components is C3, which has a molecular weight of about 187 kD and consists of an alpha and beta chain.

Typical Standard Curve

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