Rat IFN-gamma ELISA Kit


The Rat IFN-gamma ELISA Kit is an enzynme immunoassay to be used for the quantitative determination of rat Interferon-γ. The Eagle Biosciences Rat IFN-gamma ELISA Kit is intended for research use only and not for use in diagnostic or clinical procedures.

Rat IFN-gamma ELISA Kit

The Rat IFN-gamma ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 10 pg/ml
Dynamic Range: 31.25 pg/ml – 1000 pg/ml
Incubation Time: 3h45
Sample Type: Serum, Cell Culture Supernatant
Sample Size: 100 µl
Alternative Names: Interferon Gamma, IFNγ, IFNg

Assay Background

Different populations of T-cells secrete differing patterns of cytokines that ultimately lead to different immune responses. IFNγ production is a key function of Th1, CD8+ CTLs and also NK cells. It is a cytokine critical for cell mediated immunity against viral and intracellular bacterial infections and is involved in the inflammatory response following secretion via macrophage activation and stimulation of antibody secretion. IFNg is the hallmark effector cytokine of Th1 and therefore is an excellent marker for identifying a host response to intracellular pathogens.

IFNg is produced during infection by T cells of the cytotoxic/suppressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. Th1 cells secrete IL-2, IL-3, TNFα and IFNγ, whereas Th2 cells mainly produce IL-3, IL-4, IL-5, and IL-10, but little or no IFNγ. IFNγ preferentially inhibits the proliferation of Th2 but not Th1 cells, indicating that the presence of IFNγ during an immune response will result in the preferential proliferation of Th1 cells.

In addition, IFNγ has several properties related to immunoregulation. IFNγ is a potent activator of mononuclear phagocytes, and activates macrophages to kill tumor cells by releasing reactive oxygen intermediates and TNFα. IFNγ induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines. On T and B cells IFNγ promotes differentiation. It enhances proliferation of activated B cells and can act synergistically with IL-2 to increase immunoglobulin light-chain synthesis.

The role of IFNγ as a disease marker has been demonstrated for a number of different pathological situations including, viral infection, autoimmune disease, transplant rejection, diabetes and allergy.

Related Products

High Sensitive IFN gamma ELISA Assay
Human High Sensitive IFN-gamma ELISA Assay
IFN-beta ELISA Assay Kit

Additional Information

Assay Principle

The Rat IFN-γ Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for rat IFN-γ has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN-γ concentrations and unknowns are pipetted into these wells. During the first incubation, the rat Interferon-γ antigen and a biotinylated monoclonal antibody specific for rat IFN-γ are simultaneously incubated.
After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of rat IFN-γ present in the samples.


Product Documents

Product Citations