Ranibizumab ELISA Assay Kit
The Ranibizumab ELISA Assay Kit is For Research Use Only
Size: 12×8 wells
Sensitivity: <1.25 ng/mL
Standard Range: 1.25-80 ng/ml
Incubation Time: 5 hours
Sample Type: Serum, Plasma, Cell Culture Supernatants
Sample Size: 100 µL
Alternative Name: Lucentis
The method employs the competitive enzyme immunoassay technique. Antibodies to Ranibizumab are precoated onto microwells. Samples or Standards and Ranibizumab-Biotin Conjugate are pipetted into microwells and human Ranibizumab present in the sample or standard will compete with Ranibizumab-Biotin Conjugate and bound by the capture antibody. After washing microwells, HRP conjugate is pippeted and incubated. Free HRP conjugate will be removed by washing cycle.The ready to use substrate solution (TMB) is added to microwells and color develops inversely proportional to the amount of Ranibizumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Sample Preparation and Storage:
Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided. If samples are to be used for several assays, initially aliquot samples and keep at – 20°C. For Cell Culture Supernatant – If necessary, centrifuge to remove debris prior to analysis. Samples can be stored at -20°C or -80⁰C. Avoid repeated freeze-thaw cycles.