Alemtuzumab ELISA Assay Kit

$740.00

The Eagle Biosciences Alemtuzumab ELISA is used as an analytical tool for quantitative determination of Alemtuzumab in serum, plasma and cell culture supernatant.

SKU: KBI1012 Category:

Alemtuzumab ELISA Assay Kit

For Research Use Only

Size: 12×8 wells
Sensitivity: 2.5 ng/mL
Incubation Time: 1.5 hours
Sample Type: Serum, Plasma, Cell Culture Supernatants
Sample Size: 100 µL


Additional Information

Assay Background

Alemtuzumab is a drug used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma under the trade names Campath, MabCampath, and Campath-1H, and in the treatment of multiple sclerosis as Lemtrada. It is also used in some conditioning regimens for bone marrow transplantation, kidney transplantation, and islet cell transplantation. It is a monoclonal antibody that binds to CD52, a protein present on the surface of mature lymphocytes, but not on the stem cells from which these lymphocytes are derived. After treatment with alemtuzumab, these CD52-bearing lymphocytes are targeted for destruction. MabCampath was withdrawn from the markets in the US and Europe in 2012 to prepare for a higher-priced relaunch of Lemtrada aimed at multiple sclerosis.

Assay Principle

The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Alemtuzumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Alemtuzumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Alemtuzumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Alemtuzumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Manual

Product Manual