Ranibizumab mAb-based ELISA Assay
The Ranibizumab mAb-based ELISA Assay is For Research Use Only
Size: 1 x 96 wells
Sensitivity: 0.33 ng/mL
Incubation Time: 2.5 hours
Sample Type: Aqueous humour
Sample Size: 10 µL
Alternative Name: Lucentis
This Ranibizumab mAb-based ELISA is based on a Ranibizumab-specific monoclonal antibody (catcher Ab, ImmunoGuide clone 2A1). Diluted standards and samples are incubated in the microtiter plate coated with IG-2A1 mAb. After incubation, the wells are washed. A biotinylated anti-human kappa light chain monoclonal antibody (clone ImmunoGuide 7G8(Z) is added and binds to the kappa light chain part of Ranibizumab. Following incubation, wells are washed, and the horseradish peroxidase (HRP)-conjugated streptavidin is added and binds to the biotinylated 7G8(Z) mAb. Following incubation, wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Ranibizumab in the sample or standard. Results of samples can be determined by using the standard curve. Preincubation of Ranibizumab with recombinant human VEGF inhibited the reaction. Therefore, the ImmunoGuide Ranibizumab ELISA (mAb-based) measures the free form of Ranibizumab.
Storage and stability of the Ranibizumab mAb-based ELISA Assay
The kit is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sun light. The storage and stability of specimen and prepared reagents is stated in the -corresponding chapters. The microtiter strips are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2–8°C.