Quinolinic Acid Monoclonal Antibody

$775.00

The Quinolinic Acid Monoclonal Antibody has been confirmed to be highly specific and affine by competitive ELISA, the monoclonal anti- Quinolinic acid antibody 4E11-G3 was validated for immunofluorescence (IF) and immunohistochemistry (IHC) in human midbrain tissues. For Research Use Only.

SKU: IS002 Categories: ,

Quinolinic Acid Monoclonal Antibody

The Quinolinic Acid Monoclonal Antibody is For Research Use Only

Synonyms:  Pyridine-2,3-dicarboxylic acid antibody, 2,3-pyridinedicarboxylic acid antibody,  3,4-Pyridinedicarboxylic acid,  antibody Pyridine-3,4-dicarboxylic acid antibody

Immunogen:  Conjugated quinolinic acid Isotype:  IgG1 k chain

Clone:   Clone 4E11-G3

Specificity:  When tested in competitive ELISA, the anti-Quinolinic acid antibody did not show any significant cross reactivity with Picolinic and Quinaldic acid conjugates.

Concentration:  0.5 mg/mL

Size:  50 µL

Storage:  4°C


Affinity & Specificity
Competitive ELISA demonstrates that low amounts of Quinolinic acid conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Picolinic and Quinaldic acid conjugates do not affect the reaction (high specificity).

Protocols
Immunohistochemistry (IHC): Dilute at 1:100-1:1000. Perform heat antigen retrieval (pH=9) before initiating IHC staining protocol on paraffin-embedded and frozen sections
Immunofluorescence (IF): Dilute at 1:50-1:500 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate fluorescent dyes conjugated secondary antibody
Comments: Optimal working dilutions must be determined by the end-user


References

  1. Lugo-Huitrón R, Ugalde Muñiz P, Pineda B, Pedraza-Chaverrí J, Ríos C, Pérez-de la Cruz V. Quinolinic acid: an endogenous neurotoxin with multiple targets. Oxid Med Cell Longev. 2013;2013:104024. doi: 10.1155/2013/104024. Epub 2013 Sep 5. Review.
  2. Wu W, Nicolazzo JA, Wen L, Chung R, Stankovic R, Bao SS, Lim CK, Brew BJ, Cullen KM, Guillemin GJ. Expression of tryptophan 2,3-dioxygenase and production of kynurenine pathway metabolites in triple transgenic mice and human Alzheimer’s disease brain. PLoS One. 2013 Apr 22;8(4):e59749. doi: 10.1371/journal.pone.0059749. Print 2013
  3. Schwarcz R, Bruno JP, Muchowski PJ, Wu HQ. Kynurenines in the mammalian brain: when physiology meets pathology. Nat Rev Neurosci. 2012 Jul;13(7):465-77. doi: 10.1038/nrn3257.
  4. Guillemin GJ.Quinolinic acid, the inescapable neurotoxin.FEBS J. 2012 Apr;279(8):1356-65. doi: 10.1111/j.1742-4658.2012.08485.x. Epub 2012 Mar 27. Review

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Manual

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