Quinolinic Acid Monoclonal Antibody

$970.00

The Quinolinic Acid Monoclonal Antibody has been confirmed to be highly specific and affine by competitive ELISA, the monoclonal anti- Quinolinic acid antibody 4E11-G3 was validated for immunofluorescence (IF) and immunohistochemistry (IHC) in human midbrain tissues. For Research Use Only.

SKU: IS002 Categories: ,

Quinolinic Acid Monoclonal Antibody

The Quinolinic Acid Monoclonal Antibody is For Research Use Only

Synonyms:  Pyridine-2,3-dicarboxylic acid antibody, 2,3-pyridinedicarboxylic acid antibody,  3,4-Pyridinedicarboxylic acid,  antibody Pyridine-3,4-dicarboxylic acid antibody

Immunogen:  Conjugated quinolinic acid Isotype:  IgG1 k chain

Clone:   Clone 4E11-G3

Specificity:  When tested in competitive ELISA, the anti-Quinolinic acid antibody did not show any significant cross reactivity with Picolinic and Quinaldic acid conjugates.

Concentration:  0.5 mg/mL

Size:  50 µL

Storage:  4°C


Affinity & Specificity
Competitive ELISA demonstrates that low amounts of Quinolinic acid conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Picolinic and Quinaldic acid conjugates do not affect the reaction (high specificity).

Protocols
Immunohistochemistry (IHC): Dilute at 1:100-1:1000. Perform heat antigen retrieval (pH=9) before initiating IHC staining protocol on paraffin-embedded and frozen sections
Immunofluorescence (IF): Dilute at 1:50-1:500 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate fluorescent dyes conjugated secondary antibody
Comments: Optimal working dilutions must be determined by the end-user


References

  1. Lugo-Huitrón R, Ugalde Muñiz P, Pineda B, Pedraza-Chaverrí J, Ríos C, Pérez-de la Cruz V. Quinolinic acid: an endogenous neurotoxin with multiple targets. Oxid Med Cell Longev. 2013;2013:104024. doi: 10.1155/2013/104024. Epub 2013 Sep 5. Review.
  2. Wu W, Nicolazzo JA, Wen L, Chung R, Stankovic R, Bao SS, Lim CK, Brew BJ, Cullen KM, Guillemin GJ. Expression of tryptophan 2,3-dioxygenase and production of kynurenine pathway metabolites in triple transgenic mice and human Alzheimer’s disease brain. PLoS One. 2013 Apr 22;8(4):e59749. doi: 10.1371/journal.pone.0059749. Print 2013
  3. Schwarcz R, Bruno JP, Muchowski PJ, Wu HQ. Kynurenines in the mammalian brain: when physiology meets pathology. Nat Rev Neurosci. 2012 Jul;13(7):465-77. doi: 10.1038/nrn3257.
  4. Guillemin GJ.Quinolinic acid, the inescapable neurotoxin.FEBS J. 2012 Apr;279(8):1356-65. doi: 10.1111/j.1742-4658.2012.08485.x. Epub 2012 Mar 27. Review

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Package Inserts


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.