L-Kynurenine Monoclonal Antibody
The L-Kynurenine Monoclonal Antibody is For Research Use Only
Synonyms:(S)-Kynurenine antibody, L-2-Amino-4-(2-aminophenyl)-4-oxobutanoic acid antibody, Kynurenin antibody, Anthraniloyl-L-alanine antibody
Isotype:IgG1 k chain
Specificity:When tested in competitive ELISA, the anti-L-Kynurenine antibody did not show any significant cross reactivity with L-Tryptophan, 3-hydroxy-DL-Kynurenine, Kynurenic acid, Anthranilic acid or 3-hydroxyAnthranilic acid conjugates
Size: 0.5 mg/mL
Storage buffer: Before use, vial should be resuspended in 50 µL of ultrapure water. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
Immunohistochemistry (IHC): Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections
Immunofluorescence (IF): Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Before staining, perform heat antigen retrieval
Affinity & Specificity
Competitive ELISA demonstrates that low amounts of L-Kynurenine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of 3-OH-Kynurenine conjugate do not affect reaction (high specificity).
Karami, P;Majidi, MR;Johari-Ahar, M;Barar, J;Omidi, Y;, (2016). Development of screen-printed tryptophan-kynurenine immunosensor for in vitro assay of kynurenine-mediated immunosuppression effect of cancer cells on activated T-cells. Biosens Bioelectron.