Protein A (natural & structurally conserved recombinant Protein A specific) ELISA Assay Kit
The Protein A (natural & structurally conserved recombinant Protein A specific) ELISA Assay Kit is For Research Use Only
Size: 1 x 96 wells
Calibrator Range: 0 -4000 pg/mL
Sample Size: 100 uL
Sample Type: Cell Culture Supernatant and other Biological Preparations
Incubation Time: 1 hour 10 minutes
Controls Not Included
Assay Background
A variety of antibodies are used as therapeutics agent as well as in pharmaceuticals. These antibodies are generally purified through protein A column chromatography. This method of purification has the potential with contamination of protein A, which might get leached out of the column and co-eluted with purified antibody. This may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the product antibody. The highly sensitive Protein A ELISA is a quantitative, simple and will aid in monitoring the purification process as well as screening product prior to its use.
Assay Principle
This assay is based on the principle of Sandwich ELISA. Samples containing Protein A are reacted with anti-Protein A:HRP antibody simultaneously in the microtiter wells already coated with affinity purified capture anti-Protein A antibody. This immunological reaction results in formation of a sandwich complex of solid phase antibody-Protein-enzyme labeled antibody. The wells are washed to remove any unbound reactants as per the wash procedure (see Assay procedure section mentioned below). TMB substrate is then added. Color develops proportionally to the amount of Protein A in the samples. Color development is stopped by addition of stop solution. Absorbance is measured at 450 nm
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