NGAL Stool ELISA Kit
NGAL Stool ELISA Kit Developed and Manufactured in the USA
Size: 1×96 wells
Dynamic Range: 1.2 – 97.2 µg/g
Incubation Time: 2 hours
Sample Type: Stool (Also available for Plasma)
Sample Size: 50 mg Stool
Alternative Names: Neutrophil Gelatinase-Associated Lipocalin, Lipocalin-2
For Research Use Only
The NGAL ELISA Assay Kit has standards, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to human NGAL and incubated at room temperature for one hour. The plate is then washed and horseradish peroxidase (HRP) conjugated anti NGAL is added to each well. After an additional incubation period, a “sandwich” of solid-phase polyclonal antibody – human NGAL – HRP conjugated antibody” is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step.
For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction, which is terminated with an acidic reagent (i.e. ELISA stop solution). The absorbance is then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human NGAL in the test sample. A standard curve is generated by plotting the absorbance versus the respective human NGAL concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of human NGAL in test samples is determined directly from this standard curve.
The validation data of this test were generated by using Fecal Sample Collection Tube (Eagle Biosciences Cat.# CAL35-C50). Each kit contains 50 tubes. A different fecal NGAL test result may be obtained by using a different type of fecal sample collection tube.