Pembrolizumab (Keytruda) ELISA Assay Kit
The Pembrolizumab (Keytruda) ELISA Assay Kit is For Research Use Only
Size: 12 x 8 wells
Standard Range: 0 – 640 ng/ml
Sensitivity: 8.25 ng/ml
Incubation Time: 2 hours 30 minutes
Sample Type: Serum and plasma
Sample Size: 100 µL
Assay Principle
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Pembrolizumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Pembrolizumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-pembrolizumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Pembrolizumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Assay Background
Pembrolizumab, sold under the brand name Keytruda, is a humanized antibody used in cancer immunotherapy that treats melanoma, lung cancer, head and neck cancer, Hodgkin lymphoma, stomach cancer, and cervical cancer. Pembrolizumab was approved for medical use in the United States in 2014. In 2017, the US Food and Drug Administration (FDA) approved it for any unresectable or metastatic solid tumor with certain genetic anomalies (mismatch repair deficiency or microsatellite instability). It is on the World Health Organization’s List of Essential Medicines.
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