Semaglutide (Ozempic) ELISA Assay Kit


The Semaglutide (Ozempic™) ELISA Assay Kit it is used for estimation of Semaglutide in solutions and human serum. The Semaglutide ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

Semaglutide (Ozempic) ELISA Assay Kit

The Semaglutide (Ozempic) ELISA Assay Kit is For Research Use Only

Size: 12 x 8 wells
Standard Range: 0 – 3000 ng/ml
Sensitivity: 50 ng/ml
Incubation Time: 4 hours 30 minutes
Sample Type: Serum and plasma
Sample Size: 100 µL

Assay Principle

The Semaglutide ELISA is a competitive immunoassay for the determination of Semaglutide. The anti-GLP-1 antibodies are coated on 96 well plate. A constant concentration of Biotinylated GLP-1 and varying concentration of unlabeled Semaglutide or sample compete for binding to anti-GLP-1 antibodies. Captured Biotinylated GLP-1 antibodies are subsequently bound by Streptavidin-HRP which produces a soluble colored product after addition of TMB substrate. The enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of bound Semaglutide molecule present in standards or samples.

Assay Background

Semaglutide (trade name Ozempic) is a pharmaceutical drug in development by a Danish company Novo Nordisk for the treatment of type 2 diabetes. It is marketed by the name Ozempic. As a glucagon-like peptide-1 receptor agonist, it lowers the blood sugar level by increasing the production of insulin. It was discovered in 2012, by a team of researchers at Novo Nordisk as a longer-acting alternative to liraglutide. FDA approval was received in October 2017. It can be used as both injection-type or oral-type drug.

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Product Manual

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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