Benralizumab ELISA Assay Kit

$1,390.00

The Benralizumab ELISA is used as an analytical tool for quantitative determination of Benralizumab in serum, plasma and cell culture supernatant. The Benralizumab ELISA is for research use only and not to be used for diagnostic procedures.

SKU: KBI1183 Categories: , ,

Benralizumab ELISA Assay Kit

The Benralizumab ELISA Assay Kit Assay Kit is For Research Use Only

Size: 12 x 8 wells
Standard Range: 10 ng/ml-320ng/ml
Sensitivity: 7.5 ng/ml
Incubation Time: Minimum 1 hour 15 minutes
Sample Type: Serum, plasma and cell culture supernatant
Sample Size: 100 µL


Assay Principle

The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Benralizumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Benralizumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Benralizumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Benralizumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm. The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Benralizumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Benralizumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Benralizumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Benralizumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.


Sample Preparation and Storage

Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided. If samples are to be used for several assays, initially aliquot samples and keep at – 20°C.

For Cell Culture Supernatant – If necessary, centrifuge to remove debris prior to analysis. Samples can be stored at -20°C or -80⁰C. Avoid repeated freeze-thaw cycles.


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Additional Information

Assay Background


Benralizumab is a monoclonal antibody is used for the treatment of asthma. It is directed against the alpha-chain of the interleukin-5 receptor (CD125). The humanized monoclonal antibody benralizumab specifically binds to IL-5Rα, thereby preventing the interaction between IL-5 and its receptor. In addition, through its Fc constant region, benralizumab binds to the FcγIIIRa receptor expressed by natural killer cells, thus inducing eosinophil apoptosis operated by the release of proapoptotic proteins such as granzymes and perforins. Benralizumab is a monoclonal antibody is used for the treatment of asthma. It is directed against the alpha-chain of the interleukin-5 receptor (CD125). The humanized monoclonal antibody benralizumab specifically binds to IL-5Rα, thereby preventing the interaction between IL-5 and its receptor. In addition, through its Fc constant region, benralizumab binds to the FcγIIIRa receptor expressed by natural killer cells, thus inducing eosinophil apoptosis operated by the release of proapoptotic proteins such as granzymes and perforins.

Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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