OLAB Anti-Oxidized LDL ELISA

$765.00

The OLAB IgG Anti-Oxidized Low Density Lipoprotein ELISA Assay Kit is used for the quantititative determination of human IgG autoantibodies against oxidized low density lipoprotein in serum.  The Eagle Biosciences OLAB IgG Anti-Oxidized Low Density Lipoprotein ELISA Assay Kit is for Research Use Only and should not be used in diagnostics procedures.

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OLAB IgG Anti-Oxidized LDL ELISA

OLAB Anti-Oxidized LDL ELISA Developed and Manufactured in Austria by Biomedica

Size: 1×96 wells
Sensitivity: 37 mU/ml
Dynamic Range: 37 – 1,200 mU/ml
Incubation Time: 2.5 hours
Sample Type: Serum
Sample Size: 50 µL
Alternative Names: OLAB IgG Anti-Oxidized Low Density Lipoprotein ELISA, Human OLAB IgG Anti-Oxidized ELISA, Anti-Oxidized LDL Antibody ELISA
For Research Use Only

Specificity:
Anti-oxidized LDL autoantibodies

Precision:
In-between-run (n=5): ≤ 8 % CV
Within-run (n=8): ≤ 4 % CV

Normal Range:
Median: 263 mU/ml (n = 50)
Each laboratory has to establish its own reference range for the samples under investigation.

Controls Included

Assay Principle

The oLAB ELISA kit is an indirect enzyme immunoassay for the quantitative determination of anti-oxidized LDL autoantibodies in serum samples.

In a first step, prediluted standard/control/sample are pipetted into the wells of the microtiter strips, which are pre-coated with oxidized LDL antigen. Anti-oxidized LDL autoantibodies present in the standard/control/sample bind to the pre-coated antigen in the well. After a washing step, which removes all non-specific unbound material, the conjugate (monoclonal anti-human IgG-HRP) is pipetted into the wells and reacts with the anti-oxidized LDL autoantibodies.
After another washing step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells. The enzyme-catalyzed color change of the substrate is directly proportional to the amount of anti-oxidized LDL autoantibodies present in the sample. This color change is detectable with a standard microplate reader. The concentration of anti-oxidized LDL autoantibodies in the sample is determined directly from the dose response curve.

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Additional Information

Assay Background

Oxidized low density lipoprotein (oLDL) is believed to play a critical role in the development and progression of atherosclerosis. Accumulation of oLDL in macrophages and smooth muscle cells causes foam cell formation, an initial step in the disease. Autoantibodies against oxidatively modified LDL can be used as a parameter that consistently mirrors the occurrence of oxidation processes taking place in vivo. In fact, elevated levels of autoantibodies against oLDL have been detected in the blood stream of patients with coronary artery disease. Moreover, recent studies indicate a correlation between autoantibodies against oLDL and the progression of carotid atherosclerosis. Increased serum concentrations of oLAB have also been described in various diseases such as pre-eclampsia and systemic lupus erythematosus. Decreased oLAB titers were observed during septicemia and myocardial infarction.

Assay Procedure

  • Add 200 µl ASYBUF (Assay buffer) into respective wells of the coated microtiter strips, including blank
  • Add 20 µl 1:5 pre-diluted STD/SAMPLE/CTRL into each well except blank, swirl gently.  ATTENTION: The transfer of the pre-diluted STD/SAMPLE/CTRL into the coated microtiter strips must be completed within 15 minutes. Use a Multichannel pipette.
  • Cover tightly and incubate for 1.5 hours at 37°C.
  • Aspirate and wash wells 4x with 300 µl diluted WASHBUF (Wash buffer), remove remaining WASHBUF by hitting plate against paper towel after the latest wash.
  • Add 100 µl CONJ (Conjugate) into each well except blank.
  • Cover tightly and incubate for 30 minutes at room temperature (18-26°C).
  • Aspirate and wash wells 4x with 300 µl diluted WASHBUF (Wash buffer), remove remaining WASHBUF by hitting plate against paper towel after the latest wash.
  • Add 100 µl SUB (Substrate) into each well.
  • Incubate for 15 min at room temperature (18-26°C) in the dark.
  • Add 50 µl STOP (Stop solution) into each well.
  • Measure absorbance immediately at 450 nm with reference 620 nm, if available.

OLAB Anti-Oxidized LDL ELISA

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