OxyStat Colorimetric Assay Kit

$640.00

The OxyStat Colorimetric Assay is used for the quantitative determination of total peroxide concentration in human serum, plasma, or cell culture samples. The OxyStat Colorimetric Assay is for Research Use Only and should not be used in diagnostic procedures.

SKU: BI-5007 Categories: , ,

OxyStat Colorimetric Assay Kit

OxyStat Colorimetric Assay Kit Developed and Manufactured in Austria by Biomedica

Size: 1×96 wells
Sensitivity: 6 µmol/L
Standard Range: 7 – 600 µmol/l
Incubation Time: 15 minutes
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 10 µl
For Research Use Only

Controls Included


Product Overview

Colorimetric test system for the determination of the total oxidative status/capacity (TOS/TOC) in EDTA plasma, serum and other biological samples.


Assay Background

Cells and tissues are sensitive to oxidative stress, caused by the formation of free radicals. If not deactivated by antioxidants, organic peroxides and hydroperoxides are the first reaction products between cellular constituents and free radicals or other reactive oxygen derivates.  The determination of the oxidative status / oxidative stress is essential in today’s medical research and diagnostics. Methods used so far were either expensive (HPLC), or detected only degradation products of polyunsaturated fatty acids, like TBARS (thiobarbituric acid reactive substances).
The OxyStat Colorimetric Assay Kit measures the total peroxide concentration of a sample, utilizing a quick and simple assay procedure. Results show a direct correlation between free radicals and circulating biological peroxides and thus allow the characterization of the oxidative status in biological samples.
Possible Indications

  • Cardiovascular disease
  • Atherosclerosis
  • Sepsis
  • Carcinogenesis
  • Inflammatory processes
  • Neurodegenerative processes

Reagents and Sample Preparation

We recommend EDTA-plasma as sample type because in serum a time dependent increase in peroxide concentration is observed. When serum is chosen, please make sure that during preparation of serum a time period no longer than 30 min at room temperature is allowed for clotting. Store EDTA or serum samples at -20°C. Heparinized plasma should not be used for this assay, because it often precipitates upon storage and thus changes the results.
Heparin-plasma, lipemic or haemolytic samples may give erroneous results.
Cloudy samples should be centrifuged at least 5 minutes at 5000xg before use in the assay. All samples should be mixed well before assaying.


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Additional Information

Assay Principle


The peroxide concentration is determined by reaction of the biological peroxides with peroxidase and a subsequent colour reaction using TMB as substrate. After addition of a stop solution, the coloured liquid is measured photometrically at 450 nm.  A calibrator is used to calculate the concentration of circulating biological peroxides in the sample (one-point calibration).

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