Plasma Metanephrine ELISA Assay Kit

$510.00

The Eagle Biosciences Plasma Metanephrine ELISA Assay Kit is used for the quantitative determination of free Metanephrine in plasma.

Plasma Metanephrine ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 14.9 pg/ml
Standard Range: 36 – 3 600 pg/ml
Incubation Time: 5 hours 25 min
Sample Type: Plasma
Sample Size: 200 µl

Controls Included

Additional Information

Assay Background


Metanephrine and Normetanephrine are the metabolites of the catecholamines Epinephrine and Norepinephrine, respectively. Cells derived from neuroendocrine tumors (e.g. pheochromcytoma) are known to produce catecholamines which are secreted episodically via vesicles into the blood stream. But beside this, a small portion of the catecholamines is metabolized inside the cells to the corresponding catecholamines metabolites – namely Metanephrine, Normetanephrine and 3-Methoxytyramine – which are secreted at low levels continuously into the blood stream.
This kit is for research use only and should not be used as a diagnostic tool.

Assay Principle


Metanephrine (Metadrenaline) is first extracted using an ion exchange matrix followed by an acylation process. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.

Manual

Product Manual