Normetanephrine & Metanephrine (Plasma) ELISA Assay Kit
Normetanephrine & Metanephrine ELISA Developed and Manufactured in Germany by DLD Diagnostika
Size: 2 x 96 wells
Sensitivity: Metanephrine: 7 pg/ml, Normetanephrine: 7 pg/ml
Dynamic Range: Metanephrine: 20 – 3000 pg/ml, Normetanephrine: 20 – 3000 pg/ml
Incubation Time: 4 hours
Sample Type: EDTA Plasma
Sample Size: 200 µL
For Research Use Only
Controls Included
Normal Range
Metanephrine: <90 pg/ml
Normetanephrine: <190 pg/ml
It is suggested that each laboratory establish its own normal ranges for the Normetanephrine & Metanephrine ELISA
Conversion:
Metanephrine: 1 pg / ml = 5.07 pmol / l
Normetanephrine: 1 pg / ml = 5.46 pmol / l
Assay Principle
The Normetanephrine & Metanephrine ELISA Assay Kit is competitive and uses the microtiter plate format. Metanephrine and normetanephrine, respectively, are bound to the solid phase of the microtiter plate. Acylated nor-/metanephrines from the sample and solid phase bound nor-/metanephrines compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase nor-/metanephrine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.
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