Mouse Soluble alpha-Klotho ELISA


The Mouse Soluble alpha-Klotho ELISA Assay Kit is intended for the quantitation of Mouse Soluble alpha-Klotho concentrations in urine. The Eagle Biosciences Mouse Soluble alpha-Klotho ELISA Assay Kit is for research use only and not for diagnostic or therapeutic procedures.

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Mouse Soluble alpha-Klotho ELISA

Mouse Soluble alpha-Klotho ELISA Developed and Manufactured by Immuno-Biological Laboratories Co., Ltd

Size: 1×96 wells
Sensitivity: 18.06 pg/mL
Standard Range: 78.13 – 5000.0 pg/mL
Incubation Time: 2 hours
Sample Type: Urine
Sample Size: 100 µl

Assay Background

“α-klotho” was identified as an extremely down-regulated gene in the genetically-modified mouse of which phenotype is very similar to various symptoms of human aging. Then, the sequences of α-klotho genes have been identified in various species including humans based on the one of mouse. α-Klotho protein is a 130 kDa, one-transmembrane protein and its expression is confirmed in the kidneys and the parathyroid glands. In recent years, it has become clear that α-Klotho is an important molecule within a living organism regulating the metabolism of mineral such as calcium and phosphorus. Therefore, it is considered that, in α-klotho mouse, early aging-like symptoms are induced by impaired mineral homeostasis caused by decreased expression of α-Klotho.

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Additional Information

Assay Principle

This kit is a solid phase sandwich ELISA (Enzyme-linked Immunosorbent Assay). As a primary antibody is coated on a plate, samples and standard are added into the wells for 1st reaction. After the reaction, HRP-conjugated secondary antibody is added into the wells for 2nd reaction. After washing away unbound the secondary antibody, Tetra Methyl Benzidine (TMB) is added to the wells and color develops.

Typical Standard Curve

Mouse Soluble alpha-Klotho ELISA


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