Human Soluble alpha-Klotho ELISA


The Human Soluble alpha-Klotho ELISA Assay Kit is intended for the quantitation of Human Soluble alpha-Klotho concentrations in serum, plasma (EDTA, Heparin, citrate), urine and cell culture supernatant. The Eagle Biosciences Human Soluble alpha-Klotho ELISA Assay Kit is for research use only and not for diagnostic or therapeutic procedures.

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Human Soluble alpha-Klotho ELISA

Human Soluble alpha-Klotho ELISA Developed and Manufactured by Immuno-Biological Laboratories Co., Ltd

Size: 1×96 wells
Sensitivity: 6.15 pg/mL
Standard Range: 0 / 93.75 – 6000 pg/mL
Incubation Time: 2 hours
Sample Type: Serum, plasma (EDTA, Heparin, Citrate), urine and cell culture supernatant
Sample Size: 100 µl

Assay Background

α-Klotho is a key player that integrates a multi-step regulatory system of calcium metabolism and phosphate homeostasis. α-klotho was identified as an extremely down-regulated gene in the genetic mutation mouse (Klotho mouse) of which phenotype is very similar to various symptoms of human aging. Then, the sequences of α-Klotho genes have been identified in various species including human based on the one of mouse. α-Klotho protein is a 130kDa, one-transmembrane protein and its expression is confirmed in the kidneys and the parathyroid glands. In recent years, it has become clear that α-Klotho is an important molecule within a living organism regulating the metabolism of mineral such as calcium and phosphorus. Therefore, it is considered that, in Klotho mouse, early aging-like symptoms are induced by impaired mineral homeostasis caused by decreased expression of α- Klotho. Meanwhile, it is reported that the long N-terminal extracellular domain which comprises the major portion of sequence of α-Klotho protein is released free into blood by shedding. However, there are may unclear points about functions and changes in concentration of free (soluble) α-Klotho protein, so it has been required to develop the measurement system of α-Klotho. Human soluble α-Klotho protein in human blood and urine can be measured by this assay kit. Solid phase sandwich ELISA for the determination of human soluble Alpha Klotho ( α-Klotho ) in serum and EDTA-plasma. For research use only, not for use in diagnostic procedures.

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Additional Information

Assay Principle

This kit is a solid phase sandwich ELISA (Enzyme-linked Immunosorbent Assay). As a primary antibody is coated on a plate, samples and standard are added into the wells for 1st reaction. After the reaction, HRP-conjugated secondary antibody is added into the wells for 2nd reaction. After washing away unbound the secondary antibody, Tetra Methyl Benzidine (TMB) is added to the wells and color develops.

Typical Standard Curve

Human Soluble alpha-Klotho ELISA


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