GLP-1 Total ELISA Assay
GLP-1 Total ELISA Assay Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: 0.6 pmol/L
Dynamic Range: 2 -54 pmol/L
Incubation Time: overnight
Sample Type: Plasma
Sample Size: 100 µL
Alternative Names: Glucagon-like-peptide-1
For Research Use Only
The primary amino acid sequence of GLP-1 peptide is identical among mammalian species, i.e. rat, mouse, pig, human, etc. The Eagle Biosciences GLP-1 Total ELISA Assay Kit (enzyme-linked immunoassay kit) is intended quantitative determination of sum value of glucagon-like peptide 1 (7-36) and (9-36) [GLP-1 (7-36)] and (9-36) [GLP-1 (9-36)] in plasma samples. Eagle Biosciences GLP-1 Total ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
This GLP-1 Total Assay is designed, developed and produced for the quantitative measurement of GLP-1 (7-36) and (9-36) in plasma sample. The GLP-1 Total ELISA Kit utilizes the two-site “sandwich” technique with two selected GLP-1 antibodies.
Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 specific antibody and a horseradish peroxidase (HRP)-conjugated GLP-1 specific antibody is added to each well. After the first incubation period, a “sandwich” immunocomplex of “Streptavidin – Biotin-Antibody – GLP-1(7-36)/(9-36) – HRP-conjugated antibody” is formed and attached to the wall of the plate. The unbound HRP-conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36)/(9-36) on the wall of the microtiter well is directly proportional to the amount of Total GLP-1 in the sample.