Mouse Periostin ELISA Assay
The Mouse Periostin ELISA Assay is Developed and Manufactured in Austria by Biomedica
Size: 1×96 wells
Sensitivity: 0.125 nmol/l
Dynamic Range: 0-16 nmol/l
Incubation Time: 4.5 hours
Sample Type: Serum, Plasma
Sample Size: 5 µL
For Research Use Only
Controls Included
Areas of interest:
-Bone diseases
-Respiratory diseases
-Tissue repair
-Cancer diseases
-Cardiovascular diseases
Assay Principle
This kit is a sandwich enzyme immunoassay for the quantitative determination of periostin in mouse serum and plasma samples. In a first step, STD/sample/CTRL and detection antibody (goat anti mouse Periostin-Biotin) are pipetted into the wells of the microtiter strips, which are pre-coated with monoclonal mouse anti Periostin antibody. Periostin present in the sample binds to the pre-coated antibody in the well and forms a sandwich with the detection antibody. In a wash step non-specific unbound material is removed. Then conjugate (Strepatvidin-HRPO) is added and reacts with the detection antibody. After another washing step, the substrate (TMB Tetramethylbenzidine) is pipetted into the wells. The enzyme catalysed colour change of the substrate is directly proportional to the amount of mouse Periostin present in the sample. This colour change is detectable with a standard microtiter plate ELISA reader. A dose response curve of the absorbance (optical density, OD at 450 nm) vs. standard concentration is generated, using the values obtained from the standard. The concentration of mouse Periostin in the sample is determined directly from the dose response curve.
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