Mouse Periostin ELISA Assay

$640.00

The Mouse Periostin ELISA Assay is an enzyme immunoassay intended for the determination of mouse periostin in serum and plasma. The Eagle Biosciences Mouse Periostin ELISA Assay is for research use only and not to be used in diagnostic procedures.

Mouse Periostin ELISA Assay

The Mouse Periostin ELISA Assay is Developed and Manufactured in Austria by Biomedica

Size: 1×96 wells
Sensitivity: 0.125 nmol/l
Dynamic Range: 0-16 nmol/l
Incubation Time: 4.5 hours
Sample Type: Serum, Plasma
Sample Size: 5 µL

For Research Use Only

Controls Included

Areas of interest:
-Bone diseases
-Respiratory diseases
-Tissue repair
-Cancer diseases
-Cardiovascular diseases


Assay Principle

This kit is a sandwich enzyme immunoassay for the quantitative determination of periostin in mouse serum and plasma samples. In a first step, STD/sample/CTRL and detection antibody (goat anti mouse Periostin-Biotin) are pipetted into the wells of the microtiter strips, which are pre-coated with monoclonal mouse anti Periostin antibody. Periostin present in the sample binds to the pre-coated antibody in the well and forms a sandwich with the detection antibody. In a wash step non-specific unbound material is removed. Then conjugate (Strepatvidin-HRPO) is added and reacts with the detection antibody. After another washing step, the substrate (TMB Tetramethylbenzidine) is pipetted into the wells. The enzyme catalysed colour change of the substrate is directly proportional to the amount of mouse Periostin present in the sample. This colour change is detectable with a standard microtiter plate ELISA reader. A dose response curve of the absorbance (optical density, OD at 450 nm) vs. standard concentration is generated, using the values obtained from the standard. The concentration of mouse Periostin in the sample is determined directly from the dose response curve.


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Additional Information

Assay Background


Periostin (OSF-2) is secreted as a 91 kDa homodimeric soluble extracellular matrix protein expressed in collage nrich fibrous connective tissues. Periostin is involved in osteoblast recruitment, attachment and spreading. It has been associated with the epithelial-mesenchymal transition in cancer and with the differentiation of mesenchyme in the developing heart. Periostin has functions in osteology, tissue repair, oncology, cardiovascular and respiratory diseases, and in various inflammatory settings. There are at least 7 isoforms of Periostin, caused by alternative splicing (http://www.uniprot.org/uniprot/Q15063).

Typical Standard Curve


Mouse Periostin ELISA Assay

Manual

Product Manual