Neurology ELISA Assay

Mouse Myostatin ELISA Kit

$645.00

The Eagle Biosciences Mouse Myostatin/GDF8 ELISA Kit is intended for the quantitation of Mouse MSTN concentrations in cell culture supernates, tissue, serum and plasma (heparin, EDTA). The Eagle Biosciences Mouse Myostatin/GDF8 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: GDF11-K01 Categories: , ,

Mouse Myostatin ELISA Kit

The Mouse Myostatin ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 31.2pg/ml-2000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, tissue, serum and plasma(heparin or EDTA)
Sample Size: 100 µl
Alternative Name: GDF8, growth differentiation factor 8, MSTN

Assay Background

Myostatin (also known as growth differentiation factor 8, abbreviated GDF-8) is a myokine, a protein produced and released by myocytes that acts on muscle cells’ autocrine function to inhibit myogenesis: muscle cell growth and differentiation. In humans it is encoded by the MSTN gene. It is mapped to 1 C1.1; 1 26.99 cM. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.

Related Products

Rat Myostatin/GDF8 ELISA Kit
Mouse EGFR ELISA Kit
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Additional Information

Assay Principle

The Mouse MSTN Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse MSTN with a 96-well strip plate that is pre-coated with antibody specific for MSTN. The detection antibody is a biotinylated antibody specific for MSTN. The capture antibody is A monoclonal antibody from rat, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Mouse MSTN with immunogen: Expression system for standard: NSO, Immunogen sequence: D268-S376. The kit is analytically validated with ready to use reagents. To measure Mouse MSTN, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse MSTN in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse MSTN in the sample.

Typical Standard Curve

Package Insert

Product Documents

 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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