Mouse Monoclonal Anti-Desmin Antibody Clone PBM-3B4
The Mouse Monoclonal Anti-Desmin Antibody Clone PBM-3B4 is For Research Use Only
Immunogen: Full Size Recombinant Protein
Alternative Names: DES protein
Class: IgG1, Kappa
Specificity: Human, rat
Application: IHC on FFPE (formalin fixed paraffin embedded tissue sections)
Positive Control: Uterine Tissue
Demasking: Not required, temperature demasking in citrate buffer, pH 6 (12-310002) can increase the color intensity
Reagent provided: This antibody is immunoglobulin IgG1 diluted in 10 mM Phosphate buffered saline (PBS), pH 7.4 containing 1% bovine serum albumin (BSA) and 0.05% ProClin™ 300 as antimicrobial agent.
10-310038-7: Ready to Use
10-310038-01; 10-310038-05; 10-310038-1: Dilute 1:50 to 1:100 with Antibody Diluent (REF 12-310001) before use. Optimum dilution factor may vary depending on the specimen and preparation process and should be determined by each individual investigator.
Epitope retrieval: Not Required. HIER with Citrate buffer, pH 6,0 (REF 12-310002) may enhance staining in some tissues.
Staining procedure: Incubate this antibody with tissue section for 30-60 minutes at room temperature. Follow the instructions from the selected detection system.
Storage: Store at 2-8°C and in the dark. Do not use after expiration date.
Background: Antibodies binds to desmin, a protein of the intermediate filaments of all three types of muscle cells (skeletal, cardiac and smooth muscles) and tumors associated with them. Desmin is a specific marker for the myogenic differentiation of soft tissue tumor cells. Desmin is found in most rhabdomyomas, leiomyomas, rhabdomyosarcomas, leiomyosarcomas, as well as in fibroblasts. Desmin is usually absent in myoepithelial cells.
These antibodies label smooth and striated muscle cells, as well as mesothelial cells. Their use allows us to determine the subtypes of many undifferentiated and pleomorphic tumors using the analysis of intermediate filaments. Antibodies to desmin as part of diagnostic panels are used to determine various pleomorphic tumors of soft tissues and skin.
Product Developed and Manufactured by Diagomix.