Mouse Monoclonal Anti-Cytokeratin 18 Antibody Clone DC-10

Mouse Monoclonal Anti-Cytokeratin 18 Antibody Clone DC-10

The Mouse Monoclonal Anti-Cytokeratin 18 Antibody Clone DC-10 is For Research Use Only

Immunogen: Full Size Recombinant Protein
Alternative Names: KRT18, CK18
Host: Mouse
Class: IgG1, kappa
Specificity: Human
Application: IHC on FFPE (formalin fixed paraffin embedded tissue sections)
Positive Control: Gastrointestinal Tissue
Demasking: Temperature, Tris buffer, pH 9 (12-310003)
Staining: Cytoplasmic
Reagent provided: This antibody is immunoglobulin IgG1 diluted in 10 mM Phosphate buffered saline (PBS), pH 7.4 containing 1% bovine serum albumin (BSA) and 0.05% ProClin™ 300 as antimicrobial agent.
Usage dilution:
10-310011-7: Ready to Use
10-310011-01; 10-310011-05; 10-310011-1: Dilute 1:50 to 1:100 with Antibody Diluent (REF 12-310001) before use. Optimum dilution factor may vary depending on the specimen and preparation process and should be determined by each individual investigator.
Epitope retrieval: Staining of formalin fixed, paraffin embedded tissue sections is significantly enhanced by pretreatment with Tris Buffer, pH 9 (REF 12-310003).
Staining procedure: Incubate this antibody with tissue section for 30-60 minutes at room temperature. Follow the instructions from the selected detection system.
Storage: Store at 2-8°C and in the dark. Do not use after expiration date.

Background: Cytokeratin 18 is expressed in simple nonstratified epithelia. The antibody stains endothelial cells of venules, lymphatic vessels and capillaries in some tissues.

Product Developed and Manufactured by Diagomix.