Mouse FGF-21 ELISA Assay Kit
The Mouse FGF-21 ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 31.2pg/mL
Standard Range: 31.2pg/mL-2000pg/mL
Incubation Time: 2 hours 35 minutes
Sample Type: Serum, plasma, and cell culture supernate
Sample Size: 100 µl
Alternative Names: Fibroblast growth factor 21
Assay Principle
The Mouse FGF-21 assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a rabbit polyclonal antibody specific for mouse FGF-21. Standards and samples are pipetted into the wells and any mouse FGF-21 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for mouse FGF-21 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and color develops in proportion to the amount of mouse FGF-21 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse FGF-21, the unknown sample concentration can be interpolated from a reference curve included in each assay.
