Mouse Major Urinary Protein 1 (MUP-1) ELISA Assay Kit
The Mouse Major Urinary Protein 1 (MUP-1) ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.625ng/mL
Standard Range: 0.625ng/mL-40ng/mL
Incubation Time: 2 hours 15 minutes
Sample Type: Serum and plasma
Sample Size: 100 µl
Alternative Names: Major urinary protein 1, Up-1, Ltn-1, Mup-a, Lvtn-1,
Assay Principle
This assay is a sandwich ELISA designed for the quantitative detection of mouse Mup1 in samples. The immunoplate is pre-coated with antibody specific to mouse Mup1. Standards and samples are pipetted into the wells and any mouse Mup1 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for mouse Mup1 is added to the wells. After a final wash step, an HRP substrate solution is added and color develops in proportion to the amount of mouse Mup1 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse Mup1, the unknown sample concentration can be interpolated from a reference curve included in each assay.
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