Lispro NL-ELISA Assay Kit

$625.00

The Lispro NL-ELISA Assay Kit provides a method for the quantitative determination of insulin lispro in plasma or serum samples. The Lispro NL-ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: 10-1291-01 Categories: , ,

Lispro NL-ELISA Assay Kit

The Lispro NL-ELISA Assay Kit was manufactured in Sweden by Mercodia

Size: 12 x 8 wells
Sensitivity: 0.36 mU/L
Dynamic Range: 1 – 500 mU/L
Incubation Time: 2 hour 10 minutes
Sample Type: human and porcine serum, EDTA plasma and P-800 plasma
Sample Size: 10 µL


Assay Background

The Lispro NL-ELISA is a ligand binding assay able to measure insulin lispro specifically, without cross-reaction to native insulin, native proinsulin, or any of the tested insulin analogues. Also, no interference from insulin autoantibodies (IgG antibodies), which can be present in samples from diabetic patients, has been observed1. This kit is designed to fit the needs of the pharmaceutical industry, by meeting the requirements specified in the EMA2/FDA3 guidelines. Seven serum calibrators (incl. Calibrator 0), two anchor points and three controls are included in the kit.

Assay Principle

The Lispro NL-ELISA Assay Kit is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the lispro molecule. Insulin lispro in the sample reacts with anti-lispro antibodies bound to microtitration wells and peroxidase-conjugated anti-lispro antibodies in the solution. A simple washing step removes unbound enzyme-labelled antibody. The bound conjugate is detected by reaction with the chemiluminescent substrate. A chemiluminescence plate reader is used to read the intensity of light generated.


Related Products

Mouse/Rat Proinsulin ELISA Assay Kit
Proinsulin ELISA Assay Kit
Insulin ELISA Assay Kit

Additional Information

Standard Curve


Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations