L-Tryptophan Monoclonal Antibody

$970.00

The L-Tryptophan Monoclonal Antibody is validated for IHC in human brain tissues, our monoclonal anti-L-tryptophan antibody proved to work at low concentration in paraffin-embedded tissues. Highly specific, as shown by competitive ELISA, 4H11-A11 could also cater for tryptophan detection by IF.

SKU: IS011 Categories: ,

L-Tryptophan Monoclonal Antibody

The L-Tryptophan Monoclonal Antibody is For Research Use Only

Synonyms:Anti-L-Trp antibody, (S)-2-Amino-3-(3-indolyl)propionic acid antibody, L-alpha -Amino-3-indolepropionic acid antibody, (S)-2-Amino-3-(3-indolyl)propanoic acid antibody, L-alpha-Amino-3-indolepropanoic acid antibody

Immunogen:Conjugated L-Tryptophan

Isotype:IgG1 k chain

Clone:Clone 4H11-A11

Specificity: When tested in competitive ELISA, the anti-conjugated L-Tryptophan antibody did not show any significant cross reactivity with Tryptophan analogs, including Tryptamine.

Size:  0.5 mg/mL

Storage: 4°C
Storage buffer: Before use, vial should be resuspended in 50 µL of ultrapure water. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
Protocols
Immunohistochemistry (IHC): Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections
Immunofluorescence (IF): Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate.


Affinity & Specificity:
Competitive ELISA demonstrates that low amounts of Quinolinic acid conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Picolinic and Quinaldic acid conjugates do not affect the reaction (high specificity).


Background

L-Tryptophan is an essential amino acid, which is converted into Serotonin by tryptophan hydroxylase (TPH) in the gut or brain, or catabolized by various cell types into L-Kynurenine through indoleamine 2,3-dioxygenase (IDO1/2) or tryptophan 2,3-dioxygenase (TDO2). Abnormal tryptophanmetabolism is implicated in a wide range of pathological states, including metabolic and autoimmune diseases, cancer, viral infections, as well as neurodegenerative and mood disorders.


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Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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