L-Kynurenine Monoclonal Antibody
The L-Kynurenine Monoclonal Antibody is For Research Use Only
Synonyms:(S)-Kynurenine antibody, L-2-Amino-4-(2-aminophenyl)-4-oxobutanoic acid antibody, Kynurenin antibody, Anthraniloyl-L-alanine antibody
Immunogen:Conjugated L-Kynurenine
Isotype:IgG1 k chain
<Clone:Clone 3D4-F2
Specificity:When tested in competitive ELISA, the anti-L-Kynurenine antibody did not show any significant cross reactivity with L-Tryptophan, 3-hydroxy-DL-Kynurenine, Kynurenic acid, Anthranilic acid or 3-hydroxyAnthranilic acid conjugates
Size: 0.5 mg/mL
Storage: 4°C
Storage buffer: Before use, vial should be resuspended in 50 µL of ultrapure water. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
Protocols:
Immunohistochemistry (IHC): Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections
Immunofluorescence (IF): Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Before staining, perform heat antigen retrieval
Affinity & Specificity
Competitive ELISA demonstrates that low amounts of L-Kynurenine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of 3-OH-Kynurenine conjugate do not affect reaction (high specificity).
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