L-Histidine ELISA Assay Kit
L-Histidine ELISA Assay Kit Developed and Manufactured in France by ImmuSmol
Size: 1×96 wells
Sensitivity: 1.4µM (0.3 µg/mL)
Standard Range: 5.3-390µM (1.1 – 81.8µg/mL)
Incubation Time: Overnight
Sample Size: 10 uL
Sample Type: Plasma
Reactivity: Reacts with all species
Storage: Store at 2-8 °C for up to 6 months
For Research Use Only. Do not use for diagnostic
Controls Included
Specificity: No significant cross-reactivity was observed with Histamine dihydrochloride, L-Glutamic acid, 3-Methyl-L-histidine & L-Histidinol dihydrochloride
Sample Collection and Storage: EDTA Plasma. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)
Assay Principle
Enzyme Immunoassay for the quantitative determination of L-Histidine (His) in plasma samples. After extraction and derivatization L-Histidine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
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