Iodine HPLC Assay kit:
For Research Use Only
Size: 100 Tests
Incubation Time: 5 minutes
Sample Type: Urine
Sample Size: 3 mL
Product Developed and Manufactured in Germany.
Product Support in the USA.
The Eagle Biosciences Iodine HPLC Assay kit is intended for the quantitative determination of iodine in urine. The Iodine HPLC Assay Kit is for research use only and is not for use in diagnostic procedures.
For the determination of free iodine the samples are extracted on solid phase extraction cartridges. The probe is centrifuged and 50 µl of the supernatant are injected into the HPLC system. The isocratic separation via HPLC at 30°C uses a “reversed phase“column. One run lasts 10 minutes. The chromatograms are recorded by an electrochemical detector with a silver working electrode. The quantification is performed with the delivered standard; the concentration is calculated via integration of the peak areas or heights.
- The cartridge is rinsed with 3 ml methanol and 3 ml COND2. Important notice: We highly recommend not to use a centrifuge for rinsing the SPE cartridge with methanol. Methanol is highly flammable and might cause an explosion.
- 2 ml sample, CAL or CTRL are soaked through the column (alternatively a centrifuge can be used 4 min at 500 rpm). Discard the break-through.
- 1 ml sample, CAL or CTRL are soaked through the column (alternatively a centrifuge can be used 4 min at 500 rpm).
- Centrifuge the eluate and inject 50 µl in the HPLC system.
Iodine is an essential mineral which must be ingested by nutrition. The thyroid hormones T3 (triiodidethyronine) and T4 (tyroxine) are built by the addition of iodine to the amino acid tyrosine. T3 is much more effective than T4. T3 is taken up into the cells and binds to receptors of the nucleus. T3 activates a lot of metabolic pathways. It increases body temperature and oxygen consumption. T3 accelerates the ingestion of carbohydrates, activates the release of fat, and supports the turnover of cholesterol and the protein synthesis. It regulates the water balance, the bone metabolism and is indispensable for the maturation of skeleton and brain.
The measurement of free iodine in urine is a valuable tool for the determination of the supply with iodine. The measurement of the direct intake of iodine is rather difficult. Therefore the determination of iodine in urine is well accepted as an index of iodine uptake. The fecal excretion is negligible.
The Eagle Biosciences Iodine HPLC Assay kit makes it possible to determine iodine levels in an easy, fast and precise method. The Iodine HPLC Assay includes all reagents in a ready to use form for preparation and separation of the samples with exception of the columns (IC3100rp) and the controls (IC3100ko). Both can be supplied by Eagle Biosciences. Beside the complete test kits it is possible to order all components separately. Please request our single component price list.
Limitations of the Method
Serum, plasma or whole blood should not be used in the Iodine HPLC Assay kit.