Anti-Human CYP450 1A2 Goat Polyclonal Antibody

$490.00

The Anti-Human CYP450 1A2 Goat Polyclonal Antibody is for research use only and not for use in diagnostic procedures.

SKU: CYT00-A500 Categories: ,

Anti-Human CYP450 1A2 Goat Polyclonal Antibody

The Anti-Human CYP450 1A2 Goat Polyclonal Antibody is For Research Use Only

Aliquot: 0.5 mL
Storage: -20° C

Product manufactured in the USA

Product Specifications

Type: Polyclonal goat IgG .
Immunogen: Recombinant human P450 1A2 expressed in E. coli.
Specificity:
INHIBITION PROPERTIES:  Inhibits approximately 80% of the ethoxyresorufin O-deethylase activity of human liver microsomes at a concentration of 10 mg IgG/nmol P450.
WESTERN BLOTS:   recognizes a band (P450 1A2) in human liver microsomes.  Reactive with ≥ 0.2 µg of recombinant human P450 1A2.  Only slightly cross-reacts with recombinant human P450 1A1 after prolonged incubation.  Does not cross-react with recombinant human cytochromes P450 2E1, 3A4, or 2C10.
Applications: Inhibition assay or Western blots.
Contents: 0.5 mL of purified goat IgG.
Concentration: 10 mg/mL in 20 mM Phosphate buffer (pH 8.0).
Working Dilution: A dilution of 1/2000 for Western blots will yield a positive result with ≥0.2 µg of recombinant human cytochrome P450 IA2 using alkaline phosphatase-conjugated anti-goat IgG secondary antibody according to the manufacturer’s instructions.
Storage: Store at -20°C.  Do not freeze and thaw repeatedly.  Refreezing the product may result in a loss of activity.


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Additional Information

Product Specifications


Type: Polyclonal goat IgG .
Immunogen: Recombinant human P450 1A2 expressed in E. coli.
Specificity:
INHIBITION PROPERTIES:  Inhibits approximately 80% of the ethoxyresorufin O-deethylase activity of human liver microsomes at a concentration of 10 mg IgG/nmol P450.
WESTERN BLOTS:   recognizes a band (P450 1A2) in human liver microsomes.  Reactive with ≥ 0.2 µg of recombinant human P450 1A2.  Only slightly cross-reacts with recombinant human P450 1A1 after prolonged incubation.  Does not cross-react with recombinant human cytochromes P450 2E1, 3A4, or 2C10.
Applications: Inhibition assay or Western blots.
Contents: 0.5 mL of purified goat IgG.
Concentration: 10 mg/mL in 20 mM Phosphate buffer (pH 8.0).
Working Dilution: A dilution of 1/2000 for Western blots will yield a positive result with ≥0.2 µg of recombinant human cytochrome P450 IA2 using alkaline phosphatase-conjugated anti-goat IgG secondary antibody according to the manufacturer’s instructions.
Storage: Store at -20°C.  Do not freeze and thaw repeatedly.  Refreezing the product may result in a loss of activity.

Manual

Product Manual


Publications

References


Sandhu, P., Guo Z, Baba T., Martin, M.V., Tukey, R.H., and Guengerich, F.P. Arch. Biochem. Biophys. 309, 168-177, (1994).

Product Citations