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Intact MMAF ADC ELISA

$2,225.00

The Intact MMAF ADC ELISA is a highly sensitive “sandwich” test kit is intended for use in the quantitative determination of antibody MMAF conjugate level in serum or plasma of human, mouse, rat, primate, etc. in less than 2 hours. It is useful for pre-clinical and clinical pharmacology study of MMAF Antibody Drug Conjugate (ADC). The Eagle Biosciences Intact MMAF ADC ELISA Assay Kit is for research only and not used for diagnostic procedures.

SKU: MMF91-K01 Categories: , ,

Intact MMAF ADC ELISA

Intact MMAF ADC ELISA Developed and Manufactured in the USA

Size: 1×96 wells
Sensitivity: 0.40 pg/ml
Dynamic Range: 3.3 – 270.0 ng/mL
Incubation Time: 2 hours
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Names: Intact Monomethyl auristatin F Antibody Drug Conjugate, Intact MMAF Antibody Drug Conjugate, Intact Monomethyl auristatin F ADC
For Research Use Only

Assay Principle

The Intact MMAF ADC ELISA is designed, developed and produced for the quantitative measurement of antibody MMAF conjugate in serum or plasma. The assay utilizes the sandwich immunoassay technique with an antibody that binds to MMAF. Briefly, a mouse monoclonal antibody specific to MMAF is coated onto a microtiter plate. In the assay system, the assay calibrators, controls and test specimen are added to this microtiter plate. During the first incubation period, the anti-MMAF monoclonal antibody captures the MMAF-Antibody Conjugate of calibrators, controls and test samples. Unbound proteins are washed away with a wash step. A HRP (horseradish peroxidase) conjugate anti-human IgG tracer antibody is added to each well of the microtiter plate. After the second incubation, a “sandwich” immunocomplex of “Anti-MMAF antibody – MMAF Antibody Conjugate – HRP-conjugated anti-human IgG antibody” is formed and attached to the wall of the plate. The unbound HRP-conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to MMAF Antibody Conjugate on the wall of the microtiter well is directly proportional to the amount of MMAF Antibody Conjugate level in the sample.

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Additional Information

Assay Procedure

  1. Bring all reagents to room temperature (18-25°C) before use. Mix gently without causing foam.
  2. Add 25 μL of calibrators and diluted 1:100 test samples into the designated microwells. Tap the plate gently.
  3. Immediately add 100 μL of Assay Buffer
  4. Seal the plate wells securely, cover with foil or similar material to protect from light, and rotate on an ELISA plate shaker (small orbit radius) for 1 hour at 400 to 450 rpm.
  5. Wash each well 5 times by dispensing 350 μL of working wash solution into each well and then completely aspirating the contents.
  6. Alternatively, an automated microplate washer can be used.
  7. Add 100 μL of MMAF Tracer Antibody to each well. Tap the plate gently.
  8. Seal the plate wells securely, cover with foil or similar material to protect from light, and rotate on an ELISA plate shaker (small orbit radius) for 30 minutes at 400 to 450 rpm.
  9. Wash each well 5 times by dispensing 350 μL of working wash solution into each well and then completely aspirating the contents.
  10. Alternatively, an automated microplate washer can be used.
  11. Add 100 μL of ELISA HRP Substrate into each of the wells.
  12. Cover the plate with aluminum foil or similar material to avoid exposure to light. Incubate plate static, at room temperature for 20 minutes.
  13. Immediately add 100 μL of ELISA Stop Solution into each of the wells. Mix gently.
  14. Read the absorbance at 450 nm with reference filter at 620 nm.

Specificity

This Eagle Biosciences Intact MMAF ADC ELISA Assay Kit doesn’t show any cross reactivity to DM1-ADC. This assay does cross react to MMAF-ADC.

Typical Standard Curve

Intact MMAF ADC ELISA

Package Inserts

 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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