iLite TLR4 Assay Ready Cells

$1,315.00

iLite TLR4 Assay Ready Cells can be used to determine the activity of both TLR4 agonist and antagonist drugs, as well as any neutralizing antibodies against such drugs – all using the same assay. It is based on a human erythroleukemic cell line that has been genetically engineered and optimized for the quantification of LPS and for the detection of TLR4 inhibiting activity by specific and proportional expression of Firefly Luciferase. The Eagle Biosciences iLite® TLR4 Assay Ready Cells are for research use only.

iLite TLR4 Assay Ready Cells

iLite TLR4 Assay Ready Cells are Developed and Manufactured by Svar Life Science

The Eagle Biosciences iLite TLR4 Assay Ready Cells are intended for:

  • Quantification of TLR4 inhibitor activity
  • Quantification of functional LPS

Content: 250 µL of Assay Ready Cells suspended in cryoprotective medium from Gibco.
Receipt and Storage: Upon receipt confirm that adequate dry-ice is present, and the cells are frozen. Immediately transfer to -80°C storage. Cells should be stored at -80°C (do not store at any other temperature) and are stable as supplied until the expiry date shown. Cells should be used within 30 min of thawing and should be diluted immediately after thawing.
For Research Use Only


Key benefits of iLite Assays

  • Highly specific reporter gene cell lines
  • Very sensitive cell line responses (10 fold inductions)
  • Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
  • Assays within a workday (typically 4-7 hour assays)
  • Normalization gene, which eliminates unwanted matrix effects

Product Description

iLite® TLR4 Assay Ready Cells are based on the human erythromyeloblastoid leukemia cell line (K562), and have been genetically engineered and optimized to be responsive to Lipopolysaccharide (LPS) through Toll-like Receptor 4 (TLR4), resulting in a proportional expression of Firefly Luciferase. Normalization of cell counts, and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.


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Additional Information

Background


The toll-like receptor (TLR) family consist of receptors responsible for pattern recognition in innate immunity, key in the detection of pathogens and immune responses (1). The TLR4 is the most studied member of this family and induces pro-inflammatory responses upon invasion of pathogens. TLR4 is activated by binding of lipopolysaccharide (LPS, endotoxin) from Gram-negative bacteria (2). An important role of TLR4 is described in many inflammatory diseases including sepsis, asthma, cancer, acute kidney injury, or intestinal inflammation among others (1–4). Briefly, TLR4 signaling is induced upon activation in the plasmatic membrane. The signal transduction extends through TIRAP and MyD88 adaptor proteins, in early endosomes the signal pathway continues via the adaptor proteins TRAM and TRIF (2). Currently, scientist attention had been drawn to identify new molecules that can inhibit/reduce TLR4 signaling for several diseases (1,3,4).

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