Human VCAM-1 ELISA Assay


The Human VCAM-1 ELISA Assay is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of soluble Vascular Cellular Adhesion Molecule-1 (sVCAM-1) in cell culture supernatants, buffered solutions or human serum, plasma, or other body fluids. This assay will recognize both natural and recombinant human sVCAM-1. The Eagle Biosciences Human sVCAM-1/CD106 ELISA Assay kit is for Research Use Only.

SKU: C0631-K01 Categories: , ,

Human VCAM-1 ELISA Assay

The Human VCAM-1 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.6 ng/ml
Dynamic Range: 1.56 ng/ml – 50 ng/ml
Incubation Time: 1h 40min
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 100 µl
Alternative Names: CD106, soluble VCAM-1, sVCAM-1

Assay Background

The vascular cell adhesion protein 1, also known as vascular cell adhesion molecule 1 (VCAM-1) or differentiation group 106 (CD106), is a protein that in humans is coded by the VCAM1 gene. VCAM-1 functions as a cell adhesion molecule.

The VCAM-1 protein induces the adhesion of lymphocytes, monocytes, eosinophils and basophils to the vascular endothelium. It also plays a role in signal transduction of leukocyte-endothelial cells and may play a role in the development of atherosclerosis and rheumatoid arthritis.

Positive regulation of VCAM-1 in endothelial cells by cytokines results from increased transcription of the gene (eg, in response to tumor necrosis factor alpha (TNF-α) and interleukin-1 (IL-1 )) and the stabilization of the messenger RNA. (MRNA) (e.g., interleukin-4 (IL-4)). The promoter region of the VCAM-1 gene contains functional tandem sites NF-KB (nuclear factor-kappa B). The sustained expression of VCAM-1 lasts more than 24 hours.

Mainly, the VCAM-1 protein is an endothelial ligand of the VLA-4 (Very Late Antigen-4 or α4β1 integrin) of the β1 integrin subfamily. VCAM-1 expression has also been observed in other cell types (eg, smooth muscle cells). It has also been shown to interact with EZR and Moesin.

CD106 also exists on the surface of certain sub-populations of mesenchymal stem cells (MSCs).

Related Products

Human sICAM-3 ELISA Assay
Human sICAM-1 ELISA Assay Kit
Human CD102 sICAM-2 ELISA Assay

Additional Information

Assay Principle

The sVCAM-1 Kit is a solid phase sandwich Enzyme Linked Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for sVCAM-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known sVCAM-1 concentrations and unknowns are pipetted into these wells. During the first incubation, the sVCAM-1 antigen and a biotinylated monoclonal antibody specific for sVCAM-1 are simultaneously incubated.

After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of sVCAM-1 present in the samples.

  1. Add 100µl of each standard, sample and zero in duplicate to appropriate number of wells
  2. Add 50µl of diluted biotinylated anti sVCAM-1 to all wells
  3. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 1 hour
  4. Remove the cover and wash the plate
  5. Add 100μl of Streptavidin-HRP solution into all wells
  6. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 30 min
  7. Repeat wash step 5.
  8. Add 100μl of ready-to-use TMB Substrate Solution into all wells
  9. Incubate in the dark for 10-20 minutes* at room temperature. Avoid direct exposure to light by wrapping the plate in aluminium foil
  10. Add 100μl of H2SO4:Stop Reagent into all wells

Typical Standard Curve

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