Human sICAM-3 ELISA Assay
The Human sICAM-3 ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.17 ng/ml
Dynamic Range: 0.31 ng/ml – 10 ng/ml
Incubation Time: 2h 45 min
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 100 µl
Alternative Name: Human CD50 ELISA, Soluble Intercellular Adhesion Molecule 3
Specimen collection, processing & storage
Cell culture supernatants, serum, plasma or other biological samples will be suitable for use in the assay. Remove serum from the clot or red cells, respectively, as soon as possible after clotting and separation.
Cell culture supernatants:
Remove particulates and aggregates by spinning at approximately 1000 x g for 10 min.
Avoid any unintentional stimulation of the cells by the procedure. Use pyrogen/endotoxin free collecting tubes. Serum should be removed rapidly and carefully from the red cells after clotting. For that, after clotting, centrifuge at approximately 1000 x g for 10 min and remove serum.
EDTA, citrate and heparin plasma can be assayed. Spin samples at 1000 x g for 30 min to remove particulates. Harvest plasma.
If not analyzed shortly after collection, samples should be aliquoted (250-500μl) to avoid repeated freeze-thaw cycles and stored frozen at –70°C. Avoid multiple freeze-thaw cycles of frozen specimens.
The intercellular adhesion molecule 3 (ICAM 3), also called CD50 (Cluster of Differentiation 50), is a protein which, in humans, is encoded by the ICAM 3 gene.
The protein encoded by this gene belongs to the family of intercellular adhesion molecules (ICAM). All ICAM proteins are type I transmembrane glycoproteins, contain 2 to 9 immunoglobulin type C2 domains, and bind to LFA-1 leukocyte adhesion protein. This protein is constitutively and abundantly expressed by all leukocytes and may be the most important ligand for LFA-1 in initiating the immune response. It functions not only as an adhesion molecule, but also as a powerful signaling molecule.