Human CD31 ELISA Assay

$560.00

The Human CD31 ELISA is to be used for the in-vitro quantitative determination of  CD31/PECAM-1 (CD31) in human serum, plasma, buffered solutions or cell culture medium. The assay recognizes natural CD31. The Eagle Biosciences Human CD31 ELISA Assay kit is for Research Use Only.

SKU: C3131-K01 Categories: , ,

Human CD31 ELISA Assay

The Human CD31 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.17 ng/ml
Dynamic Range: 0.31 ng/ml – 10 ng/ml
Incubation Time: 2h 45 min
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 100 µl
Alternative Name: PECAM-1, Platelet endothelial cell adhesion molecule


Specimen collection, processing & storage
Cell culture supernatants, serum, plasma or other biological samples will be suitable for use in the assay. Remove serum from the clot or red cells, respectively, as soon as possible after clotting and separation.
Cell culture supernatants: Remove particulates and aggregates by spinning at approximately 1000 x g for 10 min.
Serum: Avoid any unintentional stimulation of the cells by the procedure. Use pyrogen/endotoxin free collecting tubes. Serum should be removed rapidly and carefully from the red cells after clothing. For that, after clothing, centrifuge at approximately 1000 x g for 10 min and remove serum.
Plasma: EDTA, citrate and heparin plasma can be assayed. Spin samples at 1000 x g for 30 min to remove particulates. Harvest plasma.
Storage: If not analyzed shortly after collection, samples should be aliquoted (250-500μl) to avoid repeated freeze-thaw cycles and stored frozen at –70°C. Avoid multiple freeze-thaw cycles of frozen specimens.


Assay Principle

The CD31 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD31 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD31 concentrations and unknowns are pipetted into these wells.  During the first incubation, the CD31 antigen and a biotinylated monoclonal antibody specific for CD31 are simultaneously incubated.  After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD31 present in the samples.


Related Products

Human VCAM-1 ELISA Assay
Human sICAM-1 ELISA Assay Kit
Human CD102 sICAM-2 ELISA Assay

Additional Information

Assay Background


CD31 (cluster of differentiation 31), also known as Platelet endothelial cell adhesion molecule (PECAM-1) is a glycoprotein known to be involved in cases of cell signaling and cell adhesion and a differentiation cluster protein whose gene is located on human chromosome 17.

CD31 is an important tool to study benign and malignant vascular tumors. CD31 is frequently used in measurement of angiogenesis, known to predict tumor recurrence.

Assay Procedure


  1. Add 100µl of each standard, sample, control and zero (standard diluent) in duplicate to appropriate number of wells
  2. Add 50µl of diluted biotinylated anti-CD31 to all wells
  3. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 2 hours
  4. Remove the cover and wash the plate
  5. Add 100μl of Streptavidin-HRP solution into all wells
  6. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 30 min
  7. Repeat wash step 4.
  8. Add 100μl of ready-to-use TMB Substrate Solution into all wells
  9. Incubate in the dark for 10-20 minutes* at room temperature. Avoid direct exposure to light by wrapping the plate in aluminium foil
  10. Add 100μl of H2SO4:Stop Reagent into all wells

Typical Standard Curve


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