The Human TRAIL ELISA Assay is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of TRAIL in cell culture supernatants, buffered solutions or human serum, plasma, or other body fluids. This assay will recognize both natural and recombinant human TRAIL. The Eagle Biosciences Human TRAIL/Apo2L ELISA Assay kit is for Research Use Only.

SKU: C5331-K01 Categories: , ,


The Human TRAIL ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 64 pg/ml
Dynamic Range: 93.75 – 3000 pg/ml
Incubation Time: 3h 45 min
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 100 µl
Alternative Names: Apo2L, CD253, TNF-Related Apoptosis-Inducing Ligand

Assay Background

TRAIL, TNF-Related Apoptosis-Inducing Ligand (TRAIL), is a member of the tumor necrosis factor (TNF) family, also designated as Apo-2 ligand (Apo-2L) and CD253. TRAIL is a 281-amino acid protein, related most closely to Fas/Apo-1 ligand. It is expressed at cell surface as a transmembrane protein with an extracellular region and a homotrimeric subunit structure and also exists as a soluble form

TRAIL interacts with several receptors: two pro-apoptotic death receptors DR4 (TRAIL R1) and DR5 (TRAIL R2) and three anti-apoptotic decoy receptors DcR1 (TRAIL R3), DcR2 (TRAIL R4) and osteoprotegrin (OPG). Soluble TRAIL induces apoptosis through these death receptors in many transformed cells (lymphoid as well as non-lymphoid tumor cell lines).

Because of its role in apoptosis and its stable trimer, TRAIL was described as a potential tumor-specific cancer therapeutic.

Storage Instructions
Store the TRAIL ELISA Assay kit reagents between 2 and 8°C. Immediately after use remaining reagents should be returned to cold storage (2-8°C). Expiry of the kit and reagents is stated on box front labels. The expiry of the TRAIL ELISA Assay kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, the reagent is not contaminated by the first handling.

Related Products

Human CD95 ELISA Assay
Human Perforin ELISA Assay Kit
Human CD138 /SYNDECAN-1 ELISA Assay Kit

Additional Information

Assay Principle

The TRAIL Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for TRAIL has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TRAIL concentrations and unknowns are pipetted into these wells. During the first incubation, the TRAIL antigen and a biotinylated monoclonal antibody specific for TRAIL are simultaneously incubated.

After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TRAIL present in the samples.

  1. Add 100µl of each standard, control, sample and zero in duplicate to appropriate number of wells
  2. Add 50µl of diluted biotinylated anti TRAIL to all wells
  3. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 3 hours
  4. Remove the cover and wash the plate
  5. Add 100μl of Streptavidin-HRP solution into all wells
  6. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 30 min
  7. Repeat wash step 4.
  8. Add 100μl of ready-to-use TMB Substrate Solution into all wells
  9. Incubate in the dark for 10-20 minutes* at room temperature. Avoid direct exposure to light by wrapping the plate in aluminium foil
  10. Add 100μl of H2SO4:Stop Reagent into all wells

Typical Standard Curve

Package Inserts

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