Human Perforin ELISA Assay
The Human Perforin ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 40 pg/ml
Dynamic Range: 62.5 – 2000 pg/ml
Incubation Time: 2 h 45 min
Sample Type: Supernatant, Buffered Solutions
Sample Size: 100 µl
Note for serum & plasma quantification: High and non-linear detection level is found in human serum and plasma samples. The high signal appears not dependent to the antibody pairs but probably to the matrix and/or Perforin interaction with other molecules. Consequently serum and plasma quantification is under the scientist’s responsibility and specific investigations. Remark: no detection signal was found in fetal calf serum, rat, mouse and horse serum.
Perforin is a 70kDa protein containing 534 amino acid.
Perforin is involved in the killing function by CTLs and NKs. Upon degranulation, perforin inserts itself into the target cell’s plasma membrane, forming a pore. It leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis, the cell death program.
Perforin has structural and functional similarities to complement component 6, 7, 8 and 9 (C6, C7, C8, C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis.
Its role in the immune response against tumors and virus infection has been demonstrated.