Human CDNF Mouse Monoclonal Antibody

$535.00

The Human CDNF Mouse Monoclonal Antibody is intended for research use only and not for use in diagnostic or clinical procedures. Recombinant human CDNF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Purified from cell culture supernatant.

SKU: 301-100 Categories: ,

Human CDNF Mouse Monoclonal Antibody

The Human CDNF Mouse Monoclonal Antibody is For Research Use Only

Immunogen: Human CDNF
Uniprot ID: Q49AH0
Alternative Names: ARMETL1
Clone: 7D6
Clonality: Mouse monoclonal
Class: IgG1
Reactivity: Human, mouse; others not tested
Application: ELISA, WB, IF
Purification: purified by protein G affinity chromatography
Buffer: PBS pH 7.4, with 0.1% sodium azide
Concentration: 1 mg/ml
Unit Size: 100 µg
Related Products: Mono- and polyclonal antibodies to human CDNF
Shipping: This product is shipped in non-frozen liquid form in ambient conditions
Storage: Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.
Alternative Names: Cerebral Dopamine Neurotrophic Factor


Protocol: ELISA – 1:5000 to 1:10 000;  WB – 1:1000 to 1:4000; IF – 1:100 to 1:3000; Monoclonal antibody working dilution has to be established practically for each particular antigen and assay format

Background: CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta

Product Developed and Manufactured in Germany by Icosagen


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Human CDNF Rabbit IgG Polyclonal Antibody
Human Recombinant CDNF Protein

Additional Information

 

Figure 1:

 

 

 

 

 

 

 

Western Blot testing of anti-CDNF monoclonal antibody (7D6).  Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Recombinant CDNF expressed into the supernatant of CHO cell culture medium. AB

Figure 2:

Immunofluorescence detection of human CDNF expressed in U2OS cells. CDNF was visualized using anti-CDNF antibody clone 7D6 at 1 µg/ml.  Goat ant-mouse AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for CDNF specific signal and blue for nuclei. A. CDNF-expressing U2OS cells; B. Negative control

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